Transfection Reagents
Lonza Walkersville Cell Line Nucleofector Kit R
The Cell Line Nucleofector Kit R is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:B16-F0BJCOS-7HeLaHT-29Hut-78IMR-90KG-1L6LNCaPMDBKNIH/3T3NK-92PANC-1Sf9WI-38 The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution R0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Expression Systems BestBac 2.0 Δ v-cath/chiA Baculovirus Cotransfection Kit
Expression Systems popular BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors.
Unmatched productivity and stability for BEVS
Improved production methodology can reduce need for plaque purification
Produces high titers of 5 E8 to 3 E9 IU/mL of recombinant baculovirus in P1 stock
Generates P1 stock as quickly as Bac-to-Bac and other systems
Each BestBac vial contains enough material for a minimum of five cotransfections
Suitable for other expression systems including BacMam™
Contains everything you need to perform cotransfections with your transfer vector. Included in the kit are:
• BestBac DNA
• pVL1393-lacZ control vector
• Transfection medium
Expres2 TR transfection reagent
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
Lonza Walkersville Human Monocyte Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human monocytes.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Monocytes0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Cell Line Nucleofector Kit C
The Cell Line Nucleofector Kit C is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A2058CCRF-CEMMDA-MB-453MEG-01MG-63NCI-H1299 [H1299]NS0 (ECACC)SK-BR-3T2U266B1U-937The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution C0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit L
The SE Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL SE Cell Line Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessel12 x Single use pipettes
Sigma Aldrich Fine Chemicals Biosciences X-tremeGENE 9 DNA Transfection Reagent, 0.4 mL
X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.
Sigma Aldrich Fine Chemicals Biosciences X-tremeGENE HP DNA Transfection Reagent, 0.4 mL
X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.
Cell Applications Inc Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns
Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns
Lonza Walkersville P3 Primary Cell 96-well Nucleofector Kit (960 RCT)
The P3 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies for primary cellsCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:22.5 mL P3 Primary Cell Nucleofector Solution5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)10 x 96-well Nucleocuvette Plate (20 μl)
HORIZON DISCOVERY BIOSCIENCES LIMITED DharmaFECT 1 Transfection Reagent | 5x1.5ml
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments. DharmaFECT 1 has been validated in over 35 cell types.