Transfection Reagents
Invivogen dsDNA Naked, Complexed with Transfection Reagent, 100 µg
Poly(dA:dT) is a poly(deoxyadenylic-deoxythymidylic) acid sodium salt. It is a repetitive synthetic double-stranded DNA sequence of poly(dA-dT); poly(dT-dA) and a synthetic analog of B-DNA. Poly(dA:dT) is recognized by several sensors, including DAI, LRRFIP1 and AIM2. Poly(dA:dT) is also indirectly detected by RIG-I. The activity of poly(dA:dT) is tested using the reporter cell line B16-Blue IFN/. Solubility: 2 mg/ml in physiological water. Unit Size:100 g. 86828-69-5
Agilent Technologies BL21CODONPLUS(DE3)RP C.CELLS
BL21-CodonPlus -RIL and -RP Competent Cells Correct codon bias, dramatically improves expression when codon bias is a problem, includes RIL strain for AT-rich genomes, RP strain for GC-rich genomes
Sigma Aldrich Fine Chemicals Biosciences X-tremeGENE 9 DNA Transfection Reagent, 0.4 mL
X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Boca Scientific Inc LentiBlast Premium Transduction Enhancer, 1500ul
LentiBlast Premium Transduction Enhancer is designed to increase lentiviral infection and transduction efficiency in a wide range of cell types, from CD34+ hematopoietic stem cells to primary cells or cell lines such as HT1080 or SH SY5Y. LentiBlast Premium is composed of only one reagent, allowing for a new single-step protocol. With LentiBlast Premium, neutralizing electrostatic repulsions between membranes and viral particles and enhancing viral fusion with cell membranes can occur simultaneously. Due to a favorable membrane permeable effect limiting the transmembrane potential changes, LentiBlast Premium is non-toxic and totally compatible with cell viability.
New England Biolabs, Inc. NEB® 10-beta Competent E. coli (High Efficiency) – 6 x 0.2 ml/tube
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Alkali Scientific 0.75mL GENEFECT siRNA Transfection Reagent
0.75mL GENEFECT siRNA Transfection Reagent
Active Motif Chariot™
Chariot is a revolutionary delivery reagent that quickly and efficiently transports biologically active proteins, peptides and antibodies directly into cells. The typical delivery efficiency is 60-95%. Less than two hours after delivery, live cells can be assayed to determine the effects of the introduced material, without the need for fixing. This makes Chariot an ideal tool for functional studies, including delivering inhibitory proteins, labeling organelles, screening peptide libraries and studying protein half-lives & transient complementation.
Lonza Walkersville SF Cell Line 4D-Nucleofector LV Kit XL, Catalog Number V4LC-2002, Reaction Volume Up to 20 mL, Kit Size 5 reactions, Processing Time Less than 10 min, Compatible System 4D-Nucleofector LV Unit
The Lonza SF Cell Line 4D-Nucleofector LV Kit XL is specifically designed for efficient, large-scale transfection of hard-to-transfect cell lines using the 4D-Nucleofector LV Unit. This closed-system nucleofection kit modifies up to 1 x 10^9 cells effectively, retaining cell viability. It allows variable reaction volumes and rapid processing, making it ideal for transient protein production and generating modified cell lines for various assays.
- Compatible system: 4D-Nucleofector LV Unit
- Reaction volume: Up to 20 mL
- Cell numbers supported: Up to 1 x 10^9 cells
- Processing time: Less than 10 minutes
- Kit size: 5 reactions
- Optimal cell lines: HEK293 suspension clones, CHO-S suspension clones, K-562, U-937
- Applications: Large scale transient protein production, generation of modified cell lines
- Key benefits: High transfection efficiencies, maintained cell functionality
- Storage conditions: 2 to 8 °C
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit S
The SE Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL SE Cell Line Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0),2 x 16-well Nucleocuvette Strips (20 μL)
Sigma Aldrich Fine Chemicals Biosciences Rapid DNA Ligation Kit, 1 kit
The Rapid DNA Ligation Kit covalently joins either sticky-ended or blunt-ended DNA fragments.